Fig. 9. Neighbor-joining tree showing phylogenetic relationships between innexins in Drosophila and Aedes. Innexin1 from Caenorhabditis elegans (CeInx1) is the outgroup. The tree was constructed using MEGA 3 software (Kumar et al., 2004), based on the Poisson-corrected distance estimates. In this analysis, the cumulative branch length between the node of a branch (e.g. arrow) and two genes represents the proportion of amino acids that differ between them per residue (scale bar=0.1). For example, if two genes are separated by a cumulative branch length of `0.1', then one amino-acid residue differs between them for every ten amino acids. The number at each node indicates the bootstrap score (i.e. reliability) over 1000 replicates for that node. For example, a score of `94' indicates that the node occurred in 94% of the 1000 replicates. Accession numbers for Aedes innexins are listed in Table 5. Accession numbers (GenBank) for other innexins are as follows: DrInx1, NP_524824; DrPassover, NP_728361; DrInx3, NP_524730; DrInx4, NP_648049; DrInx5, NP_573353; DrInx6, NP_572374; DrInx7, NP_788872; CeInx1, NP_741826.