Fig. 4. Phylogenies of lepidopteran LW and B opsin genes. The tree is based upon a neighbor-joining analysis of nucleotide sites using Tamura–Nei model of evolution with a correction for heterogenous patterns of nucleotide substitution among lineages. The reliability of the tree was tested using 1000 bootstrap replicates. Only bootstrap support values >50% are shown. (A) The LW opsin gene tree was reconstructed using the first and second nucleotide positions (742 sites). (B) The B opsin gene tree was reconstructed using all nucleotide positions (1055 sites). GenBank accession numbers are as follows: Agriades glandon (BRh1, DQ402502; BRh2, DQ402503); Apodemia mormo (BRh, AY587906; LWRh1, AY587907; LWRh2, AY587908); Bicyclus anynana (Blue, AY918894; LW, AY918895); Colias philodice (V, AY918899); Danaus plexippus (Blue, AY605544; LW, AY605545); Heliconius erato (Blue, AY918906; LW, AY918907); Heliconius melpomene (Blue, AY918897); Limenitis arthemis astyanax (Blue, AY918902; LWRh, DQ212962); Lycaena rubidus (LWRh, AY587901; BRh1, AY587902; BRh2, AY587903); Manduca sexta (Manop1, L78080; Manop3, AD001674); Nymphalis antiopa (Blue, AY918893); Papilio glaucus (PglRh1, AF077189; PglRh2, AF077190; PglRh3, AF067080; Blue, AF077192); Papilio xuthus (PxRh1, AB007423; PxRh2, AB007425; PxRh3, AB007425; PxRh4, AB028217); Pieris rapae (PrB, AB208675; PrV, AB208674; PrL, AB188567); Polyommatus icarus (BRh1, DQ402500; BRh2, DQ402501); Satyrium behrii (BRh1, DQ402498; BRh2, DQ402499), and Vanessa cardui (VanG, AY613986; VanB, AY613987). GenBank accession number of the newly cloned Polyommatus icarus LWRh is EU088114 (orange).