Fig. 2. (A) Copper and (B) total protein concentrations in hemolymph of L. stagnalis as a function of time. Individuals were bled at time 0 (open bars; N=54 for Cu, N=53 for proteins) and sub-groups (closed bars) were bled again at 6 h (N=8 for Cu and N=6 for proteins), 12 h (N=7 for Cu and N=8 for proteins), 1 week (N=10 for Cu and total proteins), 2 weeks (N=10 for Cu and total proteins), 5 weeks (N=10 for Cu and total proteins) and 10 weeks (N=9 for Cu and total proteins). Asterisks indicate values significantly different from initial time point values. Values are means ± s.e.m.