Fig. 6. Rhodopsin-like immunoreactivity in the pineal eye of 1.5 d.p.f. surface fish (A,B,E,F,I,J,M,N,Q,R) and Pachón cavefish (C,D,G,H,K,L,O,P,S,T) after pinealectomy or removal of bilateral eyes. (A–D) Sham operated controls. (E–H) Pinealectomy. (I–L) Removal of one bilateral eye. (M–P) Removal of both bilateral eyes. (Q–T) Failed pinealectomy. Image pairs show DIC image (left) and fluorescence images of the same specimens (right). Larvae are shown from the rostral (A–P) or left (Q–T) side. Arrowheads mark pineal eye or deleted pineal eye; arrows, deleted bilateral eye. Asterisks (far left) indicate fish of each class that exhibited a positive shadow response. Scale bar in T, 100 µm; magnification is the same in each frame.