Fig. 3. Effects of hypotaurine and thiotaurine on the viability of G. dibranchiata erythrocytes exposed to H₂S in vitro. (A–D) Epifluorescence micrographs of cells in incubation buffer that were exposed for 2 h to air (A) and 0.32% H₂S (B), and cells in incubation buffer with hypotaurine (50 mmol l^–1) or thiotaurine (5.0 mmol l^–1) that were exposed for 2 h to air (C) or 0.32% H₂S (D), followed by labeling with calcein (green) and propidium iodide (PI; red). (E–F) Quantitative analysis of calcein labeling (E) and total PI labeling (F) in cells incubated with various concentrations of hypotaurine (0–50 mmol l^–1) or thiotaurine (0–5 mmol l^–1) and exposed for 2 h to 0–3.2% H₂S. N=5. Results of statistical analysis presented in Table 1.