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Figure 5


Fig. 5. Immunolocalization of pFXYD protein (red; A and D) and Na+/K+-ATPase (NKA; green; B and E) in frozen longitudinal sections of gills of pufferfish acclimated to fresh water (FW) or seawater (SW). After double staining of the same sections, merged confocal microscope images revealed that NKA and pFXYD are colocalized (yellow in C and F) in the basolateral membrane of epithelial cells in gill filaments. F, filament. Scale bar, 50 µm.