Fig. 9. Expression profiles of proteolysis associated genes during osmotic stress in G. mirabilis gill tissue. (A) RING-finger protein mRNA (Gm_44a16). Data points were plotted and labeled as described previously. The expressions of proteolysis genes not illustrated are described in Table S1N in supplementary material. (B) Ubiquitin conjugated protein expression as determined by western blot analysis. Relative intensity and significance values were calculated as described previously. Filled stars denote significant expression during hyperosmotic stress. Open stars denote significant expression during hypo-osmotic stress. Values are shown ± s.e.m. P<0.05.