Fig. 5. Expression profiles of genes participating in mitogen activated protein kinase (MAPK) signaling cascades during osmotic stress in G. mirabilis gill tissue. (A) Receptor interacting serine/threonine protein kinase 2 mRNA (RIPK-2). (B) MAPK phosphatase 1 mRNA (MKP-1). (C) MAPK phosphatase 8 mRNA (MKP-8). Data points were plotted and labeled as described previously. (D) Phosphorylated Erk1/Erk 2 protein expression as determined by western blot analysis. Relative intensity and significance values were calculated as described previously. Filled stars denote significant expression during hyperosmotic stress. Open stars denote significant expression during hypo-osmotic stress. Values are shown ± s.e.m. P<0.05.