Fig. 3. Expression profiles of genes participating in insulin-like growth factor signaling cascades identified during osmotic stress in G. mirabilis gill tissue. (A) Insulin receptor substrate 2 mRNA (IRS-2; Gm_43o05). (B) Insulin-like growth factor binding protein 1 precursor mRNA (IGFBP-1 precursor; Gm_CC11). (C) Insulin-like growth factor binding protein 1 mRNA (IGFBP-1; Gm_CF01). (D) Prolactin receptor precursor mRNA (PRL-R precursor). (E) Suppressor of cytokine signaling 3 mRNA (SOSC-3). (F) Somatostatin receptor 5 mRNA (SS-5). Data points were plotted and labeled as described previously. In cases where a single gene was represented by multiple significant features (those proceeded by a clone ID above), the feature with the strongest P-value was illustrated. Filled stars denote significant expression during hyperosmotic stress. Open stars denote significant expression during hypo-osmotic stress. Values are shown ± s.e.m. P<0.05.