Fig. 2. Expression profiles of possible novel osmosensors in gill tissue of G. mirabilis. (A) FK506 binding protein 51 (FKBP-51) mRNA. (B) Translationally controlled tumor protein (TCTP) mRNA. Data points were plotted as the natural log of the ratio of Cy5 (experimental and control) to Cy3 (reference) divided by the mean ratio of Cy5 (control) to Cy3 (reference) for all control time points. The natural log of the ratio generated for control fish was then subtracted from all hyperosmotic (filled symbols, solid line), hypo-osmotic (open symbols, broken line) and control values at each time point (0, 1, 2, 4, 12 h post-exposure). Therefore, the expression of the control data point used in the microarray ANOVA is represented as 0 at each time point, and time=0 values have a degree of handling stress incorporated. A Tukey's post hoc test was performed to determine at which time points each gene differed significantly from controls. Filled stars denote significant expression during hyperosmotic stress. Open stars denote significant expression during hypo-osmotic stress. Values are shown ± s.e.m. P<0.05.