Fig. 1. (A) Functional classification of all annotated genes that differed significantly from control values (N=168; ANOVA P<0.05) during hyper-(filled bars) and hypo-osmotic stress (open bars) in G. mirabilis gill tissue. Designations were based upon an assigned putative role during osmotic stress as determined by data contained within the Gene Ontology database, Uniprot and the primary literature. (B) Principal components analysis (PCA) illustrating gene expression differences between hyper- and hypo-osmotic stressed G. mirabilis gill tissue. PCA was performed on conditions (hyperosmotic stress shown as filled symbols; hypo-osmotic stress shown as open symbols; time=0 and controls shown as gray symbols) using all genes that differed significantly from control values (ANOVA P<0.05) during osmotic stress. Clustering indicates similar expression profiles among conditions (x-axis PCA component 1, 49.81% of variance; y-axis PCA component 2, 18.73% of variance).