Fig. 6. Effects of silencing AamOatp on Amblyomma americanum fecundity. After feeding to repletion, ticks were incubated at 25°C for 20 days to lay eggs. Egg masses were weighed then divided by engorgement masses (EMs) to calculate the egg mass conversion ratio (EMCR). The significance of difference between the mean EMCRs of AamOatp-dsRNA (mean=0.423) and control ticks non-injected (mean=0.541, P<0.0001) and those injected with GFP-dsRNA (mean=0.515, P=0.0008) were determined using the unpaired Student's t-test. The bars in this graph represent maximum and minimum data points, as indicated in the text.