Fig. 4. Validation of RNAi-mediated silencing of AamOatp. three ticks that were injected with GFP-dsRNA or AamOatp-dsRNA were manually detached at 48 h post-attachment. These ticks were individually processed for total RNA extraction. Extracted total RNA was DNAse treated and then subjected to two-step RT-PCR. PCR products were electrophoresed on a 2% agarose gel containing 1 µg ml^–1 of ethidium bromide. The numbers represent individually treated ticks.