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Figure 2


Fig. 2. 1H NMR spectra of elephant seal muscle in vivo acquired during eupnea and apnea. 1H spectra of the deoxy-Mb proximal histidyl N{delta}H signal were recorded continuously for 25 min. Each spectrum required 1 min of signal averaging. During eupnea, the deoxy-Mb signal is not detected. Once apnea begins, the deoxy-Mb signal rises, indicating oxygen desaturation. As eupnea resumes, the signal decays rapidly, reflecting a restoration of the intracellular oxygen level.