Fig. 7. Changes in AO fluorescence following exposure of cells to Ca²⁺-free medium under steady-state conditions followed by exposure to hypertonicity then restoration of isotonic conditions, in the continuous absence of Ca²⁺. Data are means ± s.e.m. of 65 cells from four independent preparations. Data were normalized as in Fig. 3. For comparison, the control (Fig. 2A) is shown as a dotted line.