Fig. 3. Changes in AO fluorescence following exposure of cells to valinomycin (10 µmol l^–1) under steady-state conditions followed by exposure to hypertonicity then restoration of isotonic conditions, in the continuous presence of valinomycin. Data are means ± s.e.m. of 67 cells from three independent preparations. Data were normalized to the mean calculated from the first five points. For comparison, the control (Fig. 2A) is shown as a dotted line.