Fig. 2. Effect of elevation of [Ca2+]i on ENaC activity. The
top panel shows single channel currents were detected when the pipette voltage
was held at +20 mV, in a cell-attached recording. After the cell was incubated
with 1 µmol l–1 thapsigargin (TG) for 5 min, ENaC activity
were reduced as shown in the lower trace.