Fig. 2 Natural variation in nutrient store expenditure following acute food deprivation. (A) Acute food deprivation does not affect total protein levels in rovers (for^R) or sitters (for^s and for^s2). (B) Three hours of acute food deprivation significantly decreases total body lipid levels in rover but not in sitter larvae. (C) Acute food deprivation does not affect whole body carbohydrate levels despite rover larvae showing significantly lower total body carbohydrate levels compared with sitter larvae. (D) Acute food deprivation decreases hemolymph carbohydrate levels in for^R larvae but not for^s or for^s2 larvae. (E) Acute food deprivation does not affect fat body carbohydrate levels; for^R larvae have significantly less carbohydrate in their fat bodies than for^s or for^s2 larvae. Error bars indicate means ± s.e.m.