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Figure 2


Fig. 2. Immunohistochemical detection of carbonic anhydrase (CA9) (blue) protein localization in longitudinal sections of the recta of freshwater-reared Ae. aegypti (A), An. gambiae (B), Oc. taeniorhynchus (C) and An. albimanus (D). Na+/K+-ATPase (red) was used as a counterstain. CA localization did not change in larvae reared in saline water; therefore, only images of freshwater-reared larvae are shown. CA localized to the dorsal anterior rectum (DAR) cells in An. gambiae and An. albimanus and to the anterior rectum (AR) of Oc. taeniorhynchus. CA was not detected in Ae. aegypti rectum. Arrowheads indicate the junction between DAR and non-DAR cells. Arrows indicate junction between anterior and posterior recta. L, lumen; PR, posterior rectum. Scale bars: A, 150 µm; B, 75 µm; C, 149.36 µm; and D, 99.32 µm.