Fig. 5. Cuticle prints. Samples were prepared as for Fig. 4 and the material adhering to the coverslip, after nematode removal, was then observed by epifluorescence (A) or confocal (B) microscopy. Cuticle prints stained with Nile Red can be observed, leaving clear impressions of the cuticular annulations and lateral alae. Scale bars, 10 µm.