Fig. 4. D. dipsaci L4s desiccated at 50% RH, 20°C for 5 min, stained with Nile Red and observed by confocal microscopy focused on the centre (A) or surface (B) of the nematodes. Lipid droplets are associated with the surface of the cuticle, particularly with the cuticular annulations and the grooves of the lateral alae. Scale bars, 10 µm.