Fig. 5. Phosphorylation levels of cJun-N-terminal kinase (JNK) in the mantle tissue (filled cycles) and in the posterior adductor muscle (PAM; opened cycles) of submersed M. barbatus during acclimation to different water temperatures. Tissue extracts were subjected to SDS-PAGE and immunoblotted for the phosphorylated form of JNKs. Representative immunoblots are shown for each acclimation temperature. Blots were quantified by laser-scanning densitometry. Values are means ± s.e.m.; N=5 preparations from different animals. ^*P<0.05 compared with the control (0 days).