Fig. 3. Levels of Hsp70 in the posterior adductor muscle (PAM) of submersed M. barbatus during acclimation to different water temperatures. Tissue extracts were subjected to SDS-PAGE and immunoblotted for Hsp70. Representative immunoblots are shown for each acclimation temperature. Blots were quantified by laser-scanning densitometry. Values are means ± s.e.m.; N=5 preparations from different animals. Hsp72 (inducible isoform), open circles; Hsp73 (constitutive isoform), filled circles. ^*P<0.05 compared with the control (0 days).