Fig. 4. The testicular index (TI) of green-phase males increased by treatments that elevate methyl farnesoate (MF) levels. Animals were treated for 15 days and the effect on their TI was determined. (A) Green-phase males were injected on days 1, 5, and 10 with either the vehicle (N=4) or with MF (N=6). MF treatment caused a significant increase in TI (^**P<0.01). (B) Green-phase males were held in 33 ppt SW at 11°C prior to the experiment. One group of animals (N=6) was analyzed on the initial day of the experiment. Animals maintained in 33 ppt SW 11°C for 15 days (Intact, N=6) had no change in their TI whereas eyestalk ablation (ESA) animals (N=5) had a significantly elevation. Transfer of animals to 33 ppt SW at 18°C (N=6) for 15 days caused a moderate increase in TI, and transfer of animals to 5 ppt SW at 18°C (N=6) caused a larger increase in TI. Bars with different letters = P<0.05; ANOVA).