Fig. 3. Hemolymph levels of MF increase after eyestalk ablation (ESA). Animals without detectable methyl farnesoate (MF) were held in 33 ppt SW at 11°C until used. (A) All of the green-phase (N=6) and red-phase (N=5) males had detectable MF after ESA. On day 1, the amount of MF (values ± s.e.m.) was lower in green-phase males than in red-phase crabs (^***P<0.001, t-test). By day 5 the levels were similar (P>0.05, t-test). (B) Red-phase (N=8) and (C) green-phase (N=7) males were treated with 5 or 33 ppt SW at 11 or 18°C, 5 or more days after ESA. SW at 5 ppt did not significantly increase MF levels of crabs in either color phase at either temperature. SW at 18°C increased MF levels, most notably at 5 ppt (bars with different letters = P<0.01, ns = P>0.05; ANOVA).