Fig. 9. (A) Northern blotting for Dpt shows that Met is not required for induction of Dpt expression by 20E; notably, JHa methoprene in the presence of 20E is able to fully suppress Dpt expression even when Met function is silenced; pMET refers to cells transfected with Met expression vector plasmid. (B) Western blot with rabbit polyclonal antibody against MET; the western blot was performed on the same samples used in the northern blot; RNAi successfully silenced Met.