Fig. 8. Dpt promoter activity with RNAi-mediated silencing (dsRNA) directed against EcR (A), Usp (B) and Met (C). E. coli MalE, control (encoding maltose binding protein). Silencing EcR and Usp abolishes the 20E response; JHa methoprene seems to suppress the weak Dpt induction that occurs in EcR or Usp knock-down cells; however, this effect is not significant (supplementary material Table S3). In contrast, silencing Met does not impair the 20E response, and JHa is fully effective in suppressing immune induction by 20E. Results are from luciferase assays with Dpt-luc cells, immune stimulated with PGN; all JH/JHa treatments were performed in combination with 20E (means ± 1 s.e.m.).