Fig. 6. 20E blocks S2^* cell proliferation, but proliferation is unaffected by JHa methoprene; JHa treatment does not prevent the 20E-mediated block in cell proliferation. Cells were left untreated (filled triangles), or were exposed to 20E (open squares), JHa (filled squares) or both hormones (open triangles); cell counts were monitored every 24 h over the next 3 days. Shown are cell counts (in units of 10⁶ cells ml^–1 cell culture media) over time. The result shown is representative of three independent experiments (data not shown).