Fig. 5. Dpt potentiation by 20E requires at least 18 h of hormone exposure in the presence of PGN (A), but suppression by JHa methoprene is rapid and does not depend on preincubation (B). Results in A are from a northern blot, with quantification of Dpt expression normalized to that of an Rp49 control (northern blot not shown). The x-axis displays the period (in hours) during which cells were exposed to 20E; crude PGN-contaminated lipopolysaccharide (LPS) preparations were used to stimulate the immune response. Results in B are from a luciferase assay with Dpt-luc cells, immune stimulated with PGN; all JH/JHa treatments were performed in combination with 20E. The x-axis displays the different hormone treatments: no hormone, 20E only (for 24 h), or 20E (for 24 h) in the presence of JHa added to cell culture at 4, 6, 8 or 24 h prior to the luciferase assay. Means ± 1 s.e.m.