Fig. 2. Juvenile hormone analog (JHa) methoprene reduces expression of Drosomycin (Drs) in females of the Drs-GFP reporter strain DD1. (A) Uninfected (ethanol jabbed), no JHa; (B) infected (E. coli jabbed), no JHa; (C) uninfected (ethanol jabbed), JHa; (D) infected (E. coli jabbed), JHa; (E) quantification of GFP signals from images (means ± 1 s.e.m.; sample size per group, N=3). Note the strong autofluorescence in the ovaries (e.g. in D). Qualitatively similar results were obtained with a GFP reporter for Diptericin (Dpt-GFP; DIG) and in a northern blot on Dpt mRNA in y, w females (data not shown), suggesting that JH/JHa acts as a suppressor of AMP induction in vivo.