Fig. 4. Gene transcription of CaHO-1 in vivo. (A) Total RNA from different
tissues (posterior kidney, head kidney, heart, intestine, spleen, gill, brain
and liver) of goldfish under normoxia (20% O2) was analyzed by
real-time PCR. Error bars represent standard deviations obtained by measuring
each tissue from three fish. (B) Inductive transcription of CaHO-1 in
posterior kidney and gill. Total RNA from posterior kidney and gill of
goldfish subjected to normoxia (as control) and hypoxia for 24h was analyzed
by real-time PCR. Error bars represent standard deviations obtained by
measuring each tissue from three fish. The data shown have been normalized to
β-actin gene transcription. (C) Inductive transcription of
CaHO-1 in goldfish larvae. Total CaHO-1 transcripts of one
normoxic (N) and one hypoxic (H) goldfish larvae of 
1.5 cm length, from a
total of three in each group, following exposure to hypoxia (1.5%
O2) for 1.5h, were analyzed by RT-PCR. Lane M, DL2000 DNA marker
lane, kb (Takara). The data shown have been normalized to β-actin gene
transcription.