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Figure 10


Fig. 10. Cryo-SEM micrographs of the leg cuticle of different insects. (A–F) Adult Pameridea roridulae bug. Cross-fractures of (A) a freshly killed and (B) a dead, dry mirid bug show a distinct layer of epicuticular grease. (C) The layer disappears after washing bugs in cold chloroform. Top views of cuticle in (D) a freshly killed, (E) a dead, dry and (F) a dry, chloroform-washed mirid bug. (G–I) Adult Calliphora vicina fly. Cross-fractures of (G) a freshly killed, (H) dead, dry and (I) a dry, chloroform-washed C. vicina. All preparations are missing a distinct epicuticular grease layer. The arrowheads indicate epicuticular grease layer; m, microtrichia; ep, epicuticle; ex, exocuticle; en, endocuticle. Scale bars, 2 µm.