Fig. 3. Dehydroascorbate (DHA) protects mitochondrial respiration with malate as a
substrate from inhibition by sulfide. Oxygen consumption rates of mitochondria
from the body wall tissue of Arenicola marina as a function of time.
Additions (indicated by arrows) were 8 mmoll–1 malate and 1
mmoll–1 ADP. Subsequently 1 mmoll–1 DHA was
added only to the assay shown by the black line at the time marked with X. The
injection of 50 µmoll–1 sulfide inhibited malate
respiration in the absence of DHA (gray line) and, by contrast, stimulated the
oxygen consumption rate in the presence of DHA (black line).