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Figure 1


Fig. 1. Western blot analysis of (A) NKCC1a and (B) NCC in the gills of Mozambique tilapia acclimatized to freshwater (FW) or seawater (SW), and (C,D) a preabsorption test for NCC immunofluorescence staining in the yolk-sac membrane of freshwater-acclimatized tilapia embryos. The NCC-positive immunoreactivity (C) is completely abolished by preabsorbing antibody against NCC with the corresponding antigen peptide (D). Molecular mass standards are indicated (kDa). Scale bar, 10 µm.