Fig. 6. Fluxes of titratable alkalinity (J_TAlk, grey bars), total ammonia (J_Tamm, white bars) and net acidic equivalents (J_netH⁺ black bars) over a 72 h period after fish fed voluntarily on a 1% of body mass ration. Positive values indicate base excretion and negative values indicate acid excretion. Values are means ± s.e.m. (N=8). An asterisk denotes a significant difference when compared with pre-feed levels (adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method), whereas a dagger indicates a significant difference of J_TAlk and J_Tamm when compared with voluntarily fed fish (P<0.05; paired t-test).