Fig. 1. The change in (A) blood pH (solid line), plasma HCO₃^– (mmol l^–1; dashed line), and (B) the partial pressure of CO₂ (P_CO2) over the duration of the experiment in which fish were fed a 1% of body mass ration via an oesophageal catheter. Values are means ± s.e.m. (N=11). An asterisk denotes a significant difference when compared with pre-feed levels [adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method], whereas a dagger indicates a significant difference of pH when compared with voluntarily fed fish (P<0.05; paired t-test).