Fig. 5. Effects of insulin and glucose on the expression level of mRNA encoding hepatic genes. Glucokinase (GK), PK, G6Pase 1 and 2, PEPCK, FBPase and FAS mRNA levels were estimated using real-time RT-PCR. The experiment was conducted in 48 h-cultured hepatocytes of rainbow trout incubated for an additional 24 h with (Insulin, filled bars) or without (Control, hatched bars) insulin (4x10^–9 mol l^–1) and in the presence of 3 or 20 mmol l^–1 glucose (glc). Expression values are normalized with elongation factor 1 EF1)-expressed transcripts and are indicated as fold variation of 3 mmol l^–1 glucose control condition. Results are expressed as means +s.e.m. (N=6) and were analyzed by one-way ANOVA followed by Student–Newman–Keuls multiple comparison test (P<0.05). ^*Significant effect of insulin. Significant effect of glucose.