Fig. 3. Expression of hepatic nuclear factor 4 (HNF4), albumin precursor (AP), serotransferin precursor (STP), tyrosine aminotransferase (TAT), glucose transporter 2 (GLUT2) and insulin receptor (InsR) assessed by conventional RT-PCR in cell suspension (CS) and hepatocytes after 4, 24, 48 and 72 h of culture. Expected lengths of the fragments are indicated in the left margin. For InsR, a larger fragment (509 bp) was also detected.