Fig. 2. (A) Western blot analysis of Akt phosphorylation in rainbow trout liver 2, 4 and 6 h after intraperitoneal administration of insulin (I) or saline solution (S) as well as in 48 h-fasted control rainbow trout (C). p-Akt, phosphorylated form of Akt. The gel was loaded with 20 µg of total protein per lane. The figure is a representative blot. Western blots were performed on six individual samples and similar results were obtained. (B) Effects of intraperitoneal administration of insulin (I) or saline solution (S) on the level of expression of mRNA encoding hepatic genes. Pyruvate kinase (PK), glucose 6-phosphatase isoforms 1 and 2 (G6Pase 1 and 2), phosphoenolpyruvate carboxykinase (PEPCK), fructose 1,6-bisphosphatase (FBPase), carnitine palmitoyltransferase 1 isoform A and B (CPT1 A and B) and fatty acid synthase (FAS) mRNA levels were estimated using real-time RT-PCR. Expression values were normalized with elongation factor 1 (EF1)-expressed transcripts and are indicated as fold variation of the saline solution-treated group. Results are expressed as means ± s.e.m. (N=6) and were analyzed by one-way ANOVA followed by Student–Newman–Keuls comparison test. ^*Significant difference (P<0.05).