Fig. 3. Immunocytochemistry and western blot of adenylate cyclase III (AC III). (A,B) Bright-field images and immunocytochemical labeling of AC III (red) in isolated crypt olfactory receptor neurons (ORNs) counterstained with DAPI (blue). (C) Near-absence of labeling after preadsorption of the antiserum with excess antigen. Scale bar: 10 mm. (D) Immunoblot of olfactory epithelium from fish and rat. The AC III antiserum marked a band of about 130 kDa in both species. 95 µg of fish protein were loaded as opposed to 15 µg of rat protein.