Fig. 6. Anion exchange chromatography of the dialysed protein fraction from the
midgut gland homogenate of C. destructor that was precipitated by
ammonium sulphate at concentrations between 30 and 60% of saturation. Protein
sample was loaded onto a Bio-Rad DEAE column and protein concentration (mg
protein ml–1,
) and endo-β-1,4-glucanase ()
and laminarinase (
) activities (µmol reducing sugars produced
min–1 ml–1) were measured in the eluted
fractions.