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Figure 5


Fig. 5. Size exclusion chromatography of endo-β-1,4-glucanase derived from the midgut gland of G. natalis. Combined and concentrated fractions 56–70 containing endo-β-1,4-glucanase activity from the DEAE anion exchange chromatography described in Fig. 1 were loaded onto a Bio-Rad P-100 column. Protein ({square}, mg protein ml–1) and endo-β-1,4-glucanase activity ({blacktriangleup}, µmol reducing sugars produced min–1 ml–1) were measured in the fractions collected.