Fig. 13. Carbohydrates produced by the hydrolysis of laminarin when incubated with laminarinase from G. natalis (A) and C. destructor (B), and by the hydrolysis of carboxymethyl cellulose when incubated with endo-β-1,4-glucanase purified from G. natalis (C) and C. destructor (D). Hydrolysate and standards (glucose and cellobiose) were run on a silica thin layer chromatography (TLC) plate with a mobile phase of n-butanol/acetic acid/water (2:1:1, v/v/v) (Nishida et al., 2007). Carbohydrate products were visualised by spraying the TLC plate with 10% (v/v) sulphuric acid in ethanol and incubating at 120°C for 15 min (Nishida et al., 2007).