Fig. 7. Effects of light on EH release in w¹¹¹⁸xUAS-rpr flies. Pharate adults that were not destined to eclose at the time of normal LOn were collected the night prior to eclosion and their CNSs were harvested (A,E) the night prior to eclosion, (B,F) 1 h before normal LOn, (C,G) 10–20 min after LOn or (D,H) held in the dark until 10–20 min after normal LOn. (A–D) CNSs immunostained for EH were scored for level of staining. (E–H) Representative Z projections.