Fig. 5. Effects of expressing TeTxLC in the EH neurons on the LOn response and EH release. Representative results are shown for the LOn shift assay for w¹¹¹⁸xUAS-TNT-L (A–D) and EHupsxUAS-TNT-L (E–H) strains. Data are presented as described for Fig. 3. (I–L) Immunostaining for EH in CNSs from w¹¹¹⁸xUAS-TNT-L flies (I) 8–11 h prior to eclosion and (J) within 1 min after eclosion, and from EHupsxUAS-TNT-L flies (K) 8–11 h prior to eclosion and (L) within 1 min after eclosion. These are representative images of projected z-series. Arrowheads indicate the EH cell bodies located in the brain (br); large arrows, the posterior portion of the EH axon that extends down the ventral ganglion (vg); small arrows, the thoracic portion of the EH axon.