Fig. 3. Effects of varying the time of the LOn signal and of loss of the eclosion hormone (EH) neurons on the LOn response. Eclosion of (A–D) the w¹¹¹⁸xUAS-rpr control flies and (E–H) EHupsxUAS-rpr flies that lack EH neurons. Flies received one of four treatments: (A,E) light beginning at normal LOn, (B,F) held in the dark until +2 h, (C,G) light beginning at –1 h or (D,H) light beginning at –2 h, as represented by the horizontal bars at the base of each panel. Legends are as described for Fig. 1. Data from an individual representative experiment is shown.