Fig. 6. Expression of Id1, Id2 and MEF2C in adult and 5 week ST muscle and EO by RT-PCR. Agarose gel showing the expression of Id1, Id2 and MEF2C in skeletal muscle and EO from control un-operated and 5 week ST fish using RT-PCR. Id1, Id2 and MEF2C transcripts were detected in all tissues analyzed. The size of the cDNA fragments for each transcript was the same as that in Fig. 5; 1 µg of cDNA was used for PCR amplification. RT-PCR analysis of GAPDH was used as a loading control. Lanes labeled M represent the 1 kb⁺ or 25 bp (Id2) DNA ladder. Other lanes as in Fig. 3.