Fig. 5. Expression of Id1, Id2 and MEF2C in adult tissues of S. macrurus by RT-PCR. Agarose gel showing the expression of partial Id1 (304 bp), Id2 (101 bp) and MEF2C (265 bp) cDNA fragments in skeletal muscle, EO, liver, brain and heart of S. macrurus using RT-PCR. For each transcript, 1 µg of cDNA was used for the PCR amplification. RT-PCR analysis of GAPDH was used as a loading control. Lanes labeled M represent the 1 kb⁺ or 25 bp (Id2) DNA ladder. Other lanes as in Fig. 3.