Fig. 4. Expression of MyoD, myogenin, myf5 and MRF4 transcripts in muscle and EO from control fish (N=5) and fish after 5 weeks spinal transection (ST, N=5) by quantitative RT-PCR. The amount of MyoD, myogenin, myf5 and MRF4 transcript per tissue type was obtained from real-time RT-PCR experiments, and data are represented as molecules of transcript per µl of mRNA of each tissue type. Each column represents the mean ± s.e.m. Significantly different from control; ^*significantly different from control or 5 week ST muscle groups at P0.05.