Fig. 6. Three successive histological sections of the mid-membraneous part of the vocal fold of a 4-year-old elk. (A) Haemalaun-Eosin stain, (B) Elastica-van Gieson stain indicating elastic fibers in black stain, (C) trichrome stain indicating collagen fibers in blue stain. Scale bars, 5 mm. Thickness measurements of the epithelium, the vocal ligament and the thyro-arytenoid muscle were taken along the dotted line in A. (D) Schematic of the histological sections: CA, lateral cricoarytenoideus muscle; TA, thyroarytenoid muscle; CT, cricothyroid muscle; VL, vocal ligament; F, fat layer that sits on the thyroid cartilage.